For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of Trypanosoma cruzi in human serum is affected by various influences including the choice of th...e nucleic acid extraction assay. In this study, three nucleic acid extraction assays were compared regarding their influence on the sensitivity of a T. cruzi-specific real-time PCR with 62 reference sera containing T. cruzi target DNA (deoxyribonucleotide acid). More than 95% of the positive sera were correctly identified after all three nucleic acid extraction strategies with a detection rate ranging from 96.8% (60/62) for the worst assay to 100% (62/62) for the best one. A matched pairs analysis for the comparison of the cycle threshold (Ct) values obtained with the 59 reference samples with positive real-time PCR results after all three nucleic acid extraction schemes indicated differences in a range of about 3 Ct steps. Summarized, all three compared nucleic acid extraction schemes were basically suitable for T. cruzi-specific PCR from serum with some minor differences. However, in the case of low quantities of circulating parasite DNA in the serum of a patient with Chagas disease, even minor effects can make a difference in the individual diagnosis.
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Sleeping sickness is a neglected tropical disease affecting rural communities in sub-Saharan Africa. The reduction in the number of reported cases in recent years indicates that disease transmission is under control. However, many aspects of patient management still need to be improved. Undiagnosed ...patients or inappropriate treatment due to an incorrect determination of the disease stage could in fact lead to its re-emergence. There is thus a strong need for new diagnostic and staging tools to keep the disease under control and to improve the clinical care of patients. This review describes the most promising biomarkers proposed so far for the diagnosis and stage determination of patients suffering from sleeping sickness, with a particular emphasis on their translation into diagnostic tools for field applications.
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A dermatologist can often diagnose scabies by visually examining a patient’s skin from head to toe.
To make sure that a patient has scabies, a dermatologist may remove some skin. This is painless. Your dermatologist will put the skin on a glass slide and look at the slide under a microscope. If y...our dermatologist sees scabies mites or their eggs, it is certain that you have scabies.
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Accessed online August 2018
Accessed online August 2018
Accessed online August 2018
Este guião tem como objetivos melhorar a qualidade do aconselhamento a doentes com HIV/SIDA, providenciar meios para garantir uma avaliação psicossocial e identificar fatores de risco que contribuem para uma baixa adesão à TARV e estabeceler a ligação entre a unidade sanitária e a comunidade....
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Hilfe und Information in der Ulmer Onlineklinik | Accessed Online January 2019. | The CATS questionnaire is a short freely accessible screening instrument directly based on the DSM-5 criteria for Posttraumatic Stress Disorder (PTSD). It is a measure of potentially traumatic events and of posttraumat...ic stress symptoms.
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Ziehl-Neelsen & Fluorescence Methods.
2nd edition
large File: 20 MB
This document aims to provide advice on the detection and management of ill travellers suspected of COVID-19 infection, at international airports, ports and ground crossings. It includes the following measures; 1)Detection of ill travellers; 2) Interview of ill travellers for COVID-19; 3) Reporting ...of alerts of ill travellers with suspected COVID-19 infection and 4) Isolation, initial case management and referral of ill travellers with suspected COVID-19 infection.
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Verlässliche Antworten und konkrete Informationen, wie Sie sich schützen und anderen helfen können
Aufklärungsplattform des Bundeministerium für Gesundheit
PERC has developed a dashboard on Tableau to visualize multidisciplinary data and guide decision-makers on their country’s current performance of the COVID-19 response.
My COVID Pass
In October 2020, the African Union officially launched the Trusted Travel platform as part of the overall Trusted Travel Initiative during a joint ministerial meeting of the ministers of health, transport, and information and communication of African Union Member States.
The data on the coronavirus pandemic is updated daily
Die neuen Podcast-Folgen werden dienstags gegen 17 Uhr veröffentlicht. Neben Christian Drosten, dem Leiter der Virologie an der Berliner Charité, ist Sandra Ciesek, Direktorin des Instituts für Medizinische Virologie am Universitätsklinikum Frankfurt am Main, ein regelmäßiger Gesprächsgast.
...Seit Ende Februar 2020 beantwortet Prof. Dr. Christian Drosten (Leiter der Virologie an der Berliner Charité) Fragen zur aktuellen Situation, erklärt Zusammenhänge und schildert, wie er diese Monate persönlich erlebt. Anfang September kam Prof. Dr. Sandra Ciesek (Leiterin der Virologie des Universitätsklinikums Frankfurt) als Gesprächsgast dazu.
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