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For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the ... of Trypanosoma cruzi in human serum is affected by various influences including the choice of the nucleic acid extraction assay. In this study, three nucleic acid extraction assays were compared regarding their influence on the sensitivity of a T. cruzi-specific real-time PCR with 62 reference sera containing T. cruzi target DNA (deoxyribonucleotide acid). More than 95% of the positive sera were correctly identified after all three nucleic acid extraction strategies with a detection rate ranging from 96.8% (60/62) for the worst assay to 100% (62/62) for the best one. A matched pairs analysis for the comparison of the cycle threshold (Ct) values obtained with the 59 reference samples with positive real-time PCR results after all three nucleic acid extraction schemes indicated differences in a range of about 3 Ct steps. Summarized, all three compared nucleic acid extraction schemes were basically suitable for T. cruzi-specific PCR from serum with some minor differences. However, in the case of low quantities of circulating parasite DNA in the serum of a patient with Chagas disease, even minor effects can make a difference in the individual diagnosis.
Adapted from CURRENT Medical Diagnosis & Treatment 2010
Guidelines for Diagnosis and Management of Lung Cancer in Indonesia
Evaluation and Diagnosis of Penicillin Allergy for Healthcare Professionals
Protocol for laboratory diagnosis of COVID_19, in the framework of the health emergency
Guidelines for the diagnosis and treatment of COVID-19 in intensive care units
Guidelines for diagnosis, acute care and post-COVID-19 follow-up for children and adolescents
Barriers to the prompt and effective diagnosis and treatment of malaria exist at both the community and health facility level. Household surveys measure malaria case management at the population level with standard indicators that assess treatment-s...
Molecular methods for antimicrobial resistance (AMR)diagnostics to enhance the Global Antimicrobial Resistance Surveillance System
Despite the development of point-of-care diagnostic tests for syphilis, chlamydia, gonorrhoea, and trichomoniasis, none comply with all WHO criteria. This analysis overviews landscape analyses of point-of-care ... technologies for Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis and syphilis, available and in the pipeline. The target audience for the target product profiles is broad and includes clinicians, researchers working on diagnostics, laboratory experts, including, microbiologists and virologists, public health experts, epidemiologists, developers, and representatives for manufactures, including biotech engineers, policy-and decision-makers as well as representatives from regulatory bodies and agencies, donor agencies and international organizations.
Pulmonary tuberculosis predictors and rapid molecular diagnosis
Preditores de tuberculose pulmonar e experiência com o diagnóstico molecular rápido
The key areas covered are diagnosis, imaging, pathology, surgery, rehabilitation, palliative care and survivorship. It emphasizes a multi-disciplinary team approach which is paramount for quality cancer care. The specific cancers co...
The internationally recognized criteria for diagnosis of neurocysticercosis include a requirement for neuroimaging techniques, such as computerized tomography (CT) and/or magnetic resonance imaging (MRI), ideally supported by serology. These facilit...
Early breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up
Guidelines and Consensus for the Diagnosis, Management and Prevention of Type 2 Diabetes Mellitus in Indonesia
Use of antibiotics in patients with a diagnosis of SARS-COV2 (Update May 2021)
Drugs, Diagnostics, Vaccines, Preventive Technologies, Research toward a cure, and immune-based and gene therapies in development
La récente augmentation du nombre de cas de microcéphalie et d’autres troubles neurologiques potentiellement associés à une infection à virus Zika a engendré une recrudescence des demandes de dépistage en laboratoire de cette infection. Les groupes prioritaires pour un test de ... doivent être constitués de personnes symptomatiques et de femmes enceintes asymptomatiques potentiellement exposées au virus Zika.
Ce document fournit des indications sur les stratégies actuelles de dépistage de l’infection à virus Zika. Il sera revu et actualisé à mesure que des informations supplémentaires deviendront disponibles.
The modules (1-12) are based on materials originally developed by FIND, KNCV and Cepheid, and are in PowerPoint format for country customization. Depending on the audience, modules may be selected and adapted according to need (e.g. basic users, supervisors, clinicians). Topics covered include: Over...
Guidance on TB and TB/HIV prevention, diagnosis, treatment and care in the workplace
