The decontamination of instruments and medical devices plays a very important role in the prevention of health care-associated infections (HAIs). Indeed, improper decontamination of surgical instruments, endoscopic devices, respiratory care devices and reusable haemodialysis devices still occurs in many settings, leading to HAIs. This course is based on the WHO manual on decontamination and reprocessing of medical devices for health care facilities, as well as in collaboration with the US CDC.