This report describes the “Building health workers capacity on air pollution and health” pilot workshop held in Ghana in 2022 which aimed at testing the training material of the first WHO Air Pollution and Health Training toolkit (APHT) targeting health professionals. APHT aims at strengthening the knowledge of health workers on air pollution and health and to enable them to effectively communicate with patients and communities on how to reduce their risk, to advocate for population level interventions as well as to train other peers and colleagues using a train-the-trainer approach. This workshop report serves as a tool and example of a training that can be replicated and adapted to other contexts and settings based on country and regional priorities and needs.